Recombinant Human IL3RA Protein (Thr19-Arg305), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10454
Official Symbol
IL3RA
Product Overview
Biotinylated recombinant human IL-3R alpha protein (Thr19-Arg305) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Thr19 and Lys20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 61 kDa
SDS-PAGE: 83-93 kDa, under reducing conditions.
Protein length
Thr19-Arg305
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-3 is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag binds with an ED50 of 3.00-45.0 ng/mL.
Endotoxin
<0.50 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-3 is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag binds with an ED50 of 3.00-45.0 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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