IMP-10609

IL4

Recombinant human IL-4 protein (His25-Ser153) with an N-terminal Met was expressed in E. coli, manufactured and tested under cGMP guidelines, and produced using non-animal reagents in an animal-free laboratory.

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

E. coli

Human

Tag Free

Met-His25-Lys-(Cys)-Asp-Ile-Thr-Leu-Gln-Glu

Lyophilized from a 0.2 μm filtered solution in PBS.

Predicted Molecular Mass: 15.1 kDa
SDS-PAGE: 14 kDa, reducing conditions

His25-Ser153

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of recombinant human IL-4 is >1.0 x 107 IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656).

<0.10 EU/μg of the protein by the LAL method.

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitute at 100-200 μg/mL in PBS.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of recombinant human IL-4 is >1.0 x 107 IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656).