Recombinant Human IL4RA protein, C-hFc tag
Product Description
Cat
IMP-1173
Official Symbol
IL4RA
Product Overview
Recombinant Human IL4RA(P24394)(Met26-Gln231) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Human
Tag
C-hFc tag
Form
Lyophilized from sterile PBS, pH 7.4.

Molecular Mass
The protein has a calculated MW of 50.2kDa, and migrates as 60-70 kDa under reducing conditions.
Protein length
Met26-Gln231
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Loaded Human IL-4RA-Fc on Protein A Biosensor, can bind Human IL-4 with an affinity constant of 0.99 nM as determined in BLI assay.

Bioactivity-BLI 2
Bioactivity-BLI

Loaded Human IL-4RA-Fc on Protein A Biosensor, can bind Human IL-4-His with an affinity constant of 0.99 nM as determined in BLI assay.

Bioactivity-Cell based assay 1
Bioactivity

Measured by its ability to inhibit IL-4-dependent proliferation of TFb1 human erythroleukemic cells. The ED50 for this effect is 5-20 ng/ml.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info