Recombinant Human IL4RA protein, C-His tag

Product Description

Cat

IMP-1172

Official Symbol

IL4RA

Product Overview

Recombinant Human IL4RA(P24394)(Met26-His232 ) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 24.4kDa, and migrates as 40-60 kDa under reducing conditions.

Protein length

Met26-His232

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-BLI 1

Loaded Human IL-4-Fc on Protein A Biosensor, can bind Human IL-4-His with an affinity constant of 0.78 nM as determined in BLI assay.

Bioactivity-BLI 2

Loaded Human IL-4RA-His on HIS1K Biosensor, can bind Human IL-4 with an affinity constant of 0.48 nM as determined in BLI assay.

Bioactivity-Cell based assay 1

Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 5-20 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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