Recombinant Human IL4RA protein, C-His tag

Product Description

Cat

IMP-1172

Official Symbol

IL4RA

Product Overview

Recombinant Human IL4RA(P24394)(Met26-His232 ) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 24.4kDa, and migrates as 40-60 kDa under reducing conditions.

Protein length

Met26-His232

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-BLI 1

Loaded Human IL-4-Fc on Protein A Biosensor, can bind Human IL-4-His with an affinity constant of 0.78 nM as determined in BLI assay.

Bioactivity-BLI 2

Loaded Human IL-4RA-His on HIS1K Biosensor, can bind Human IL-4 with an affinity constant of 0.48 nM as determined in BLI assay.

Bioactivity-Cell based assay 1

Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 5-20 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry