Recombinant Human IL5 Protein (Ile20-Ser134)
Product Description
Cat
IMP-10478
Official Symbol
IL5
Product Overview
Recombinant human IL-5 protein (Ile20-Ser134) without tag was expressed in Sf 21 cells.
Description
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2. This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Expression System
Sf 21 cells
Species
Human
Tag
Tag Free
Predicted N Terminal
Ile20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 13 kDa (monomer)
SDS-PAGE: 13-15 kDa, reducing conditions
Protein length
Ile20-Ser134
Bio-activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.04-0.2 ng/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.04-0.2 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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