Recombinant Human IL6 protein, N-His-GST Tag

Product Description

Cat

IMP-2918

Official Symbol

IL6

Product Overview

Recombinant Human IL6 protein(P05231)(Pro29~Met212), fused with N-terminal His and GST Tag, was expressed in E. coli.

Expression System

E. coli

Species

Human

Tag

N-His-GST Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

21kDa

Protein length

Ala117~Ser269

Purity

> 95%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

Effect of Human IL-6 on MMP-10 protein levels in A549 cells

Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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