Recombinant Human IL6 protein, N-His-GST Tag

Product Description

Cat

IMP-2918

Official Symbol

IL6

Product Overview

Recombinant Human IL6 protein(P05231)(Pro29~Met212), fused with N-terminal His and GST Tag, was expressed in E. coli.

Expression System

E. coli

Species

Human

Tag

N-His-GST Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

21kDa

Protein length

Ala117~Ser269

Purity

> 95%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

Effect of Human IL-6 on MMP-10 protein levels in A549 cells

Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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