Description
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]
Storage
The lyophilized IL-6, although stable at room temperature for 3 weeks, is best stored desiccated at -20 centigrade. Reconstituted Il-6 should be stored in working aliquots at -20 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Reconstitution
The lyophilized IL-6 is soluble in water and most aqueous buffers. It should be reconstituted in water to a concentration not less than 0.1 mg/mL. This solution can then be stored at 2-8 centigrade for future use or diluted into other buffered solutions. Further dilutions should be made into buffer containing carrier protein or medium containing serum.
