Recombinant Human IL6R Protein, C-hFc-tagged
Product Description
Cat
IMP-4756
Official Symbol
IL6R
Product Overview
Recombinant human IL6R (NP_000556.1) (Met1-Pro365) was expressed with the Fc region of human IgG1 at the C-terminus.
Description
Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.
Expression System
HEK293
Species
Human
Tag
C-hFc
Predicted N Terminal
Leu 20
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human IL6R consists of 584 amino acids and predicts a molecular mass of 65.3 kDa. As a result of glycosylation, it migrates as an approximately 70-100 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Pro365
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured by its ability to enhance the activity of IL6 on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 30-125 ng/mL in the presence of 100 ng/mL of recombinant human IL6.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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