Recombinant Human IL6R Protein, C-hFc&AVI-tagged, Biotinylated
Product Description
Cat
IMP-4753
Official Symbol
IL6R
Product Overview
Recombinant human IL6R (NP_000556.1) (Met1-Pro356) was expressed with a c-terminal AVI tagged Fc region of human IgG1 tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Description
Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.
Expression System
HEK293
Species
Human
Tag
C-hFc&AVI
Predicted N Terminal
Leu 20
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human IL6R consists of 599 amino acids and predicts a molecular mass of 67.1 kDa.
Protein length
Met1-Pro356
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 85 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human IL-6 at 2 μg/mL (100 μL/well) can bind Human IL-6R (ECD, hFc&AVI Tag), Biotinylated, the EC50 is 300-1200 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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