Recombinant Human IL6R Protein (Leu20-Pro365), C-6×His and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10608
Official Symbol
IL6R
Product Overview
Biotinylated recombinant human IL-6R alpha protein (Leu20-Pro365) with 6×His tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been identified in this gene. A pseudogene of this gene is found on chromosome 9.
Expression System
CHO
Species
Human
Tag
C-6×His and Avi
Predicted N Terminal
Leu20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 42 kDa
SDS-PAGE: 65-80 kDa, under reducing conditions
Protein length
Leu20-Pro365
Bio-activity
Measured by its binding ability in a functional ELISA. When Human IL-6 is immobilized at 0.25 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-6R alpha His-tag Avi-tag binds with an ED50 of 0.1-1 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 1 mg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Human IL-6 is immobilized at 0.25 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-6R alpha His-tag Avi-tag binds with an ED50 of 0.1-1 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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