Recombinant Human ITGAX&ITGB2 Protein, C-His-tagged

Product Description

Cat

IMP-4773

Official Symbol

ITGAX&ITGB2

Product Overview

Recombinant extracellular domain (Met1-Pro1107) of human ITGAX (P20702) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Asn700) of human ITGB2 (P05107), constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Description

Integrin alpha-V&beta-3 (ITGAV/ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Phe 20&Gln 23

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of human ITGAX&ITGB2 comprises 1859 (1140+719) amino acids and has a calculated molecular mass of 204.8 (125.62+79.18) kDa.

Protein length

Met1-Pro1107 and Met1-Asn700

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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