Recombinant Human KAT2A Protein (726-837 aa), His/FLAG-tagged
Product Description
Cat
IMP-6461
Official Symbol
KAT2A
Product Overview
The peptide corresponding to amino acids 726 - 837 that contains the bromodomain sequences of KAT2A (GCN5) (accession number NM_021078.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.4 kDa. It shows binding specificity for acetylated H2AK5, H3K9, H3K14, H3K9/K14, H4K8/K16, H4K16 and H4K5/8/12/16.
Description
GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. KAT2A (GCN5) acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests KAT2A (GCN5) may play a role in the induction of chromatin remodeling of proviral genes. KAT2A (GCN5) also functions as a repressor of NFkB by promoting ubiquitination of the NFkB subunit RelA in a HAT-independent manner. KAT2A (GCN5) contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Expression System
E. coli
Species
Human
Tag
His/FLAG
Form
Recombinant KAT2A (GCN5) (726-837) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol.
Molecular Mass
19.4 kDa
Protein length
726-837
Purity
>90%
Applications
Binding assays, inhibitor screening, and selectivity profiling
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
SDS-PAGE
SDS-PAGE

GCN5 (726-837) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW:19.4 kDa Purity: > 90%

Bioactivity-ELISA 1
Activity 1

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity. 3 μM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

Bioactivity-ELISA 2
Activity 2

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity. 3 μM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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