Recombinant Human KAT2A Protein (726-837 aa), His/FLAG-tagged

Product Description

Cat

IMP-6461

Official Symbol

KAT2A

Product Overview

The peptide corresponding to amino acids 726 - 837 that contains the bromodomain sequences of KAT2A (GCN5) (accession number NM_021078.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.4 kDa. It shows binding specificity for acetylated H2AK5, H3K9, H3K14, H3K9/K14, H4K8/K16, H4K16 and H4K5/8/12/16.

Description

GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. KAT2A (GCN5) acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests KAT2A (GCN5) may play a role in the induction of chromatin remodeling of proviral genes. KAT2A (GCN5) also functions as a repressor of NFkB by promoting ubiquitination of the NFkB subunit RelA in a HAT-independent manner. KAT2A (GCN5) contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.

Expression System

E. coli

Species

Human

Tag

His/FLAG

Form

Recombinant KAT2A (GCN5) (726-837) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol.

Molecular Mass

19.4 kDa

Protein length

726-837

Purity

>90%

Applications

Binding assays, inhibitor screening, and selectivity profiling

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

SDS-PAGE

GCN5 (726-837) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW:19.4 kDa Purity: > 90%

Bioactivity-ELISA 1

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity. 3 μM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

Bioactivity-ELISA 2

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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