Recombinant Human KDR Protein (Ala20-Glu764), C-Fc and 6×His-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10018

Official Symbol

KDR

Product Overview

Alexa Fluor 488 conjugated recombinant human VEGFR2/KDR protein (Ala20-Glu764) with a human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515–545 nm

Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc and 6×His

Predicted N Terminal

Ala20

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 110 kDa (monomer)
SDS-PAGE: 150-170 kDa, under reducing conditions.

Protein length

Ala20-Glu764

Bio-activity

Measured by flow cytometry for its ability to bind Human VEGFR2/KDR/Flk-1 Antibody conjugated fluorescent beads at 2.50-10.0 μg/mL (100 μL/well).
Please Note: Optimal dilutions should be determined by each laboratory for each application.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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