Recombinant Human KDR Protein (Ala20-Glu764), C-hFc and 6×His-tagged

Product Description

Cat

IMP-9774

Official Symbol

KDR

Product Overview

Recombinant human VEGFR2/KDR protein (Ala20-Glu764) with a Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc and 6×His

Predicted N Terminal

Ala20

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 110 kDa (monomer)
SDS-PAGE: 160-170 kDa, reducing conditions

Protein length

Ala20-Glu764

Bio-activity

Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-40 ng/mL in the presence of 10 ng/mL recombinant human VEGF165.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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