IMP-4336

KIT

Recombinant human KIT isoform 2 (P10721-2) cytoplasmic domain (Thr 540-Val 972) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.

Insect cell

Human

N-His&N-GST

Gln 26

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 3mM DTT, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human KIT(aa 540-972) /GST chimera consists of 670 amino acids and predictes a molecular mass of 76.8 kDa. It migrates as an approximately 68 KDa band in SDS-PAGE under reducing conditions.

Thr540-Val972

< 1.0 EU/μg of the protein as determined by the LAL method

> 92 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.