Recombinant Human KLRK1 Protein, N-His-tagged

Product Description

Cat

IMP-4851

Official Symbol

KLRK1

Product Overview

Recombinant human NKG2D (P26718-1) (Phe78-Val216) was fused with a polyhistide tag at the N-terminus.

Description

KLRK1 (Killer Cell Lectin Like Receptor K1) is a Protein Coding gene. NKG2D, also known as CD314, is an immune receptor that consists of two disulfide-linked type II transmembrane proteins with short intracellular proteins incapable to transduce signals. To transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D-therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.

Expression System

Insect cell

Species

Human

Tag

N-His

Predicted N Terminal

His

Form

Lyophilized from sterile 20mM Tris, 300mM NaCl, 10%glycerol, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human NKG2D consists of 157 amino acids and has a calculated molecular mass of 18.4 kDa. As a result of glycosylation, the apparent molecular mass of human NKG2D is approximately 22.6 kDa in SDS-PAGE under reducing conditions.

Protein length

Phe78-Val216

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized human His-NKG2D (78-216) at 2 μg/mL (100 μL/well) can bind human ULBP1-Fch, The EC50 of human ULBP1-Fch is 8-50 ng/mL.

Bioactivity-ELISA 2

Immobilized human His-NKG2D (78-216) at 10 μg/mL (100 μL/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 15-60 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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