Recombinant Human LAG3 Protein (Leu23-Leu450), C-6×His-tagged
Product Description
Cat
IMP-9551
Official Symbol
LAG3
Product Overview
Recombinant human LAG-3 (Leu23-Leu450), with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Leu23
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 47 kDa
SDS-PAGE: 57-63 kDa, reducing conditions
Protein length
Leu23-Leu450
Bio-activity
Measured by its ability to induce TNF-alpha secretion by JAWSII mouse immature dendritic cells. The ED50 for this effect is 0.2-1.2 μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce TNF-alpha secretion by JAWSII mouse immature dendritic cells. The ED50 for this effect is 0.2-1.2 μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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