Recombinant Human LAG3 Protein (Leu23-Leu450), C-Fc-tagged, Biotinylated conjugated

Product Description

Cat

IMP-10088

Official Symbol

LAG3

Product Overview

Biotinylated recombinant human LAG-3 protein (Leu23-Leu450) with a human IgG1 (Pro100-Lys330) Fc tag was expressed in Mouse myeloma cell line.

Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Leu23

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 72.7 kDa
SDS-PAGE: 74-91 kDa, reducing conditions

Protein length

Leu23-Leu450

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LSECtin/CLEC4G is coated at 1 μg/mL, 100 μL/well, biotinylated recombinant LAG-3 Fc Chimera binds with an ED50 of 0.2-2 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer, biotinylated via amines.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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