Recombinant Human LAG3 Protein (Leu23-Leu450), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10070

Official Symbol

LAG3

Product Overview

Biotinylated recombinant human LAG-3 protein (Leu23-Leu450) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Leu23

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 75 kDa
SDS-PAGE: 81-92 kDa, under reducing conditions

Protein length

Leu23-Leu450

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Hepassocin/FGL1 His-tag is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human LAG-3 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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