Recombinant Human LAG3 Protein (Leu23-Leu450), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10070

Official Symbol

LAG3

Product Overview

Biotinylated recombinant human LAG-3 protein (Leu23-Leu450) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Leu23

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 75 kDa
SDS-PAGE: 81-92 kDa, under reducing conditions

Protein length

Leu23-Leu450

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Hepassocin/FGL1 His-tag is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human LAG-3 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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