1 μg/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue Lane 1: reducing conditions Lane 2: non-reducing conditions
Human PBMCs were allowed to migrate to human LGALS3 at (0, 0.01, 0.1, 1, 10 and 100 μg/mL). After 1 hour, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above.