Recombinant Human LGALS9 Protein (Ala2-Thr323), Tag Free
Product Description
Cat
IMP-9711
Official Symbol
LGALS9
Product Overview
Recombinant human Galectin-9 protein (Ala2-Thr323) without tag was expressed in E. coli.
Description
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.
Expression System
E. coli
Species
Human
Tag
Tag Free
Predicted N Terminal
Ala2
Form
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.
Molecular Mass
Predicted Molecular Mass: 35.8 kDa
SDS-PAGE: 34 kDa, reducing conditions
Protein length
Ala2-Thr323
Bio-activity
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 500 ng/mL can bind Recombinant Human TIM-3 Fc Chimera with an apparent Kd <30 nM.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Monomer
Storage
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in water.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 500 ng/mL can bind Recombinant Human TIM-3 Fc Chimera with an apparent Kd <30 nM.

Data Sheet MSDS
Datasheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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