Recombinant Human LGALS9 Protein (Ala2-Thr323), Tag Free

Product Description

Cat

IMP-9711

Official Symbol

LGALS9

Product Overview

Recombinant human Galectin-9 protein (Ala2-Thr323) without tag was expressed in E. coli.

Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.

Expression System

E. coli

Species

Human

Tag

Tag Free

Predicted N Terminal

Ala2

Form

Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.

Molecular Mass

Predicted Molecular Mass: 35.8 kDa
SDS-PAGE: 34 kDa, reducing conditions

Protein length

Ala2-Thr323

Bio-activity

Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 500 ng/mL can bind Recombinant Human TIM-3 Fc Chimera with an apparent Kd <30 nM.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Monomer

Storage

12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in water.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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