Recombinant Human LGR4 Protein (Ala25-Thr544), C-hFc-tagged
Product Description
Cat
IMP-9894
Official Symbol
LGR4
Product Overview
Recombinant human Lgr4/GPR48 Fc Chimera protein (Ala25-Thr544) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Ala25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 83.8 kDa (monomer)
SDS-PAGE: 100-130 kDa, reducing conditions
Protein length
Ala25-Thr544
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 is coated at 0.5 μg/mL, Recombinant Human Lgr4/GPR48 Fc Chimera binds with an apparent KD <10 nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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