Recombinant Human LGR4 Protein (Ala25-Thr544), C-hFc-tagged

Product Description

Cat

IMP-9894

Official Symbol

LGR4

Product Overview

Recombinant human Lgr4/GPR48 Fc Chimera protein (Ala25-Thr544) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body.

Expression System

CHO

Species

Human

Tag

C-hFc

Predicted N Terminal

Ala25

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 83.8 kDa (monomer)
SDS-PAGE: 100-130 kDa, reducing conditions

Protein length

Ala25-Thr544

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 is coated at 0.5 μg/mL, Recombinant Human Lgr4/GPR48 Fc Chimera binds with an apparent KD <10 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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