Recombinant Human LGR5 Protein (Gly22-Ile560), C-hFc-tagged
Product Description
Cat
IMP-9909
Official Symbol
LGR5
Product Overview
Recombinant human Lgr5/GPR49 protein (Gly22-Ile560) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Gly22
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 87 kDa (monomer)
SDS-PAGE: 90-110 kDa, reducing conditions
Protein length
Gly22-Ile560
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 is coated at 0.5 μg/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent Kd <0.1 nM. Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.5-7.5 μg/mL in the presence of 20 ng/mL Recombinant Human R-Spondin 2 and 5 ng/mL Recombinant Mouse Wnt-3a.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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