Recombinant Human LGR6 Protein (Ala25-Ala567), C-hFc-tagged
Product Description
Cat
IMP-9918
Official Symbol
LGR6
Product Overview
Recombinant human Lgr6 protein (Ala25-Ala567) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. The encoded protein is a glycoprotein hormone receptor with a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding. Alternative splicing of this gene results in multiple transcript variants.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Ala25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 87 kDa
SDS-PAGE: 90-108 kDa, reducing conditions
Protein length
Ala25-Ala567
Bio-activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>80%, by SDS-PAGE with silver staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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