Recombinant Human LILRB4 protein, C-Fc Tag
Product Description
Cat
IMP-059
Official Symbol
LILRB4
Product Overview
Recombinant Human LILRB4 protein(AA Gln 22-Glu 259)(Accession # AAH26309) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-Fc Tag
Form
Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.0, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.9 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Gln 22-Glu 259
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human LILRB4, Fc Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR 1
Bioactivity-SPR

Human LILRB4, Fc Tag (SPR verified) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human ANGPTL7, His tag with an affinity constant of 71.6 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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