Recombinant Human LILRB4 protein, C-Fc Tag

Product Description

Cat

IMP-059

Official Symbol

LILRB4

Product Overview

Recombinant Human LILRB4 protein(AA Gln 22-Glu 259)(Accession # AAH26309) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.0, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.9 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 22-Glu 259

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human LILRB4, Fc Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR 1

Human LILRB4, Fc Tag (SPR verified) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human ANGPTL7, His tag with an affinity constant of 71.6 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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