Recombinant Human LILRB4 Protein (Pro17-His257), C-6×His-tagged
Product Description
Cat
IMP-9921
Official Symbol
LILRB4
Product Overview
Recombinant human LILRB4/CD85k/ILT3 protein (Pro17-His257) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Pro17& Ala (N+3 of signal derived sequence)
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 29-43 kDa, reducing conditions
Protein length
Pro17-His257
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB4/CD85k/ILT3 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 binds with an apparent Kd <1 nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB4/CD85k/ILT3 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 binds with an apparent Kd <1 nM.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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