Recombinant Human LRRC32 Protein (Met1-Asn627), C-6×His-tagged

Product Description

Cat

IMP-9851

Official Symbol

LRRC32

Product Overview

Recombinant human LRRC32/GARP protein (Met1-Asn627) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.

Description

This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies.

Expression System

CHO

Species

Human

Tag

C-6×His

Predicted N Terminal

Ala18

Form

Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.

Molecular Mass

Predicted Molecular Mass: 67.0 kDa (monomer)
SDS-PAGE: 70-80 kDa, reducing conditions

Protein length

Met1-Asn627

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LRRC32/GARP is immobilized at 5 μg/mL (100 μL/well) Recombinant Human Latent TGF-beta 1 binds with an apparent Kd <30 nM.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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