Recombinant Human LRRC32 Protein (Met1-Asn627), C-6×His-tagged

Product Description

Cat

IMP-9851

Official Symbol

LRRC32

Product Overview

Recombinant human LRRC32/GARP protein (Met1-Asn627) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.

Description

This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies.

Expression System

CHO

Species

Human

Tag

C-6×His

Predicted N Terminal

Ala18

Form

Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.

Molecular Mass

Predicted Molecular Mass: 67.0 kDa (monomer)
SDS-PAGE: 70-80 kDa, reducing conditions

Protein length

Met1-Asn627

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LRRC32/GARP is immobilized at 5 μg/mL (100 μL/well) Recombinant Human Latent TGF-beta 1 binds with an apparent Kd <30 nM.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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