Recombinant Human MADCAM1 Protein (Val23-Gln317), C-6×His and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10060
Official Symbol
MADCAM1
Product Overview
Biotinylated recombinant human MAdCAM-1 protein (Val23-Gln317) with 6×His tag and Avi tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.
Expression System
HEK293
Species
Human
Tag
C-6×His and Avi
Predicted N Terminal
Val23
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 34 kDa
SDS-PAGE: 65-75 kDa, under reducing conditions.
Protein length
Val23-Gln317
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha 4 beta 7 Protein is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human MAdCAM-1 His-tag Avi-tag binds with an ED50 of 0.150-1.50 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha 4 beta 7 Protein is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human MAdCAM-1 His-tag Avi-tag binds with an ED50 of 0.150-1.50 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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