Recombinant Human MAP2K6 Protein, N-His&N-GST-tagged
Product Description
Cat
IMP-4830
Official Symbol
MAP2K6
Product Overview
Recombinant human MKK6 isoform 1 (P52564-1) (Met 1-Asp 334) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Description
Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.
Expression System
Insect cell
Species
Human
Tag
N-His&N-GST
Predicted N Terminal
Met
Form
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
Molecular Mass
The recombinant human MKK6/GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Asp334
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 80 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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