Recombinant Human MAP2K6 Protein, N-His&N-GST-tagged
Product Description
Cat
IMP-4830
Official Symbol
MAP2K6
Product Overview
Recombinant human MKK6 isoform 1 (P52564-1) (Met 1-Asp 334) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Description
Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.
Expression System
Insect cell
Species
Human
Tag
N-His&N-GST
Predicted N Terminal
Met
Form
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
Molecular Mass
The recombinant human MKK6/GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Asp334
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 80 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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