Recombinant Human MAP3K5 Protein (649-946), N-GST-tagged
Product Description
Cat
IMP-9773
Official Symbol
MAP3K5
Product Overview
Recombinant human Active ASK1 (aa 649-946) protein with a N-GST tag was expressed in Spodoptera frugiperda, Sf 9 cells.
Description
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Expression System
Sf 9 cells
Species
Human
Tag
N-GST
Form
Supplied in 50 mM Tris HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Molecular Mass
SDS-PAGE: 60 kDa
Protein length
649-946
Bio-activity
The specific activity of ASK1 is typically 82-112 nmol/min/mg using a myelin basic protein (MBP) substrate.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain at 5 μg per lane.
Storage
This product is stable at ≤ -70 centigrade for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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