Recombinant Human MDM2 Protein , Tag Free
Product Description
Cat
IMP-10096
Official Symbol
MDM2
Product Overview
Recombinant human MDM2/HDM2 protein without tag was expressed in E. coli.
Description
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Expression System
E. coli
Species
Human
Tag
Tag Free
Form
X mg/ml (X μM) in 50 mM Hepes pH 8.0, 200 mM NaCl, 10% glycerol, 5 mM TCEP
Molecular Mass
Predicted Molecular Mass: 55 kDa
Bio-activity
Recombinant Human Mdm2/Hdm2 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Mdm2/Hdm2 concentration of 0.5-5 μM.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
Email
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