Recombinant Human MERTK protein, C-His Tag
Product Description
Cat
IMP-6058
Official Symbol
MERTK
Product Overview
Recombinant Human MERTK protein(Q12866)(21-505aa), fused with C-terminal 10xHis tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-His Tag
Form
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 0.2 M Arg, 6% Trehalose, pH 8.0
Molecular Mass
55.4 kDa
Protein length
21-505aa
AA Sequence
AITEAREEAKPYPLFPGPFPGSLQTDHTPLLSLPHASGYQPALMFSPTQPGRPHTGNVAIPQVTSVESKPLPPLAFKHTVGHIILSEHKGVKFNCSISVPNIYQDTTISWWKDGKELLGAHHAITQFYPDDEVTAIIASFSITSVQRSDNGSYICKMKINNEEIVSDPIYIEVQGLPHFTKQPESMNVTRNTAFNLTCQAVGPPEPVNIFWVQNSSRVNEQPEKSPSVLTVPGLTEMAVFSCEAHNDKGLTVSKGVQINIKAIPSPPTEVSIRNSTAHSILISWVPGFDGYSPFRNCSIQVKEADPLSNGSVMIFNTSALPHLYQIKQLQALANYSIGVSCMNEIGWSAVSPWILASTTEGAPSVAPLNVTVFLNESSDNVDIRWMKPPTKQQDGELVGYRISHVWQSAGISKELLEEVGQNGSRARISVQVHNATCTVRIAAVTRGGVGPFSDPVKIFIPAHGWVDYAPSSTPAPGNADPVLII
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized MERTK at 2 μg/mL can bind anti-MERTK antibody(CSB-RA621519A1HU), the EC50 is 32.95-48.25 ng/mL.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized MERTK at 2 μg/ml can bind anti-MERTK antibody(CSB-RA621519A1HU), the EC50 is 32.95-48.25 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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