Recombinant Human MICA (AAH16929.1) extracellular domain (Glu24-Gln308) protein, fused with a polyhistidine tag at the C-terminus, was expreesed in HEK293 Cells.
Expression System
HEK293
Species
Human
Tag
C-His tag
Form
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human MICA consists of 296 amino acids and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human MICA is approximately 45-65 kDa due to glycosylation.
Protein length
Glu24-Gln308
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 97% as determined by SDS-PAGE
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
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