IMP-1936

MS4A1

Recombinant human MS4A1 (NP_068769.2) (Ile141-Ser188) was expressed with a TrxA tag at the N-terminus. The purified protein was biotinylated in vitro.

CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed in all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule that plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells are usually in the normal range but can be very low.

E. coli

Human

N-TrxA

Met

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human MS4A1 consists of 218 amino acids and predicts a molecular mass of 23.9 kDa.

Ile141-Ser188

Please contact us for more information.

> 85 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Using the Octet RED System, the affinity constant (Kd) of CD20 Protein, Human, Recombinant (TrxA Tag), Biotinylated bound Mabthera was 0.04 μM.