Recombinant Human MSLN Protein (Arg35-Leu289), C-6×His-tagged
Product Description
Cat
IMP-9558
Official Symbol
MSLN
Product Overview
Recombinant human Mesothelin protein (Arg35-Leu289) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Arg35
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 28 kDa
SDS-PAGE: 27-39 kDa, reducing conditions
Protein length
Arg35-Leu289
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin is immobilized at 4 μg/mL (100 μL/well), the concentration of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag that produces 50% of the optimal binding response is 3-18 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Monomer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin is immobilized at 4 μg/mL (100 μL/well), the concentration of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag that produces 50% of the optimal binding response is 3-18 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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  • 1-631-559-9269
    1-516-512-3133
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