Recombinant Human MSLN Protein (Glu296-Gly580), C-6×His-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10015

Official Symbol

MSLN

Product Overview

Alexa Fluor 488 conjugated recombinant human Mesothelin protein (Glu296-Gly580) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-6×His

Predicted N Terminal

Glu296

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 33 kDa
SDS-PAGE: 40-45 kDa, reducing conditions.

Protein length

Glu296-Gly580

Bio-activity

Measured by flow cytometry for its ability to bind anti-human Mesothelin Monoclonal Antibody conjugated beads. The concentration of Recombinant Human Mesothelin C-Terminal (aa 296-580) His-tag Alexa Fluor 488 that produces 50% of the binding response is 25.0-250 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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