Recombinant Human MUC1 Protein (Ser24-Ser380), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10037
Official Symbol
MUC1
Product Overview
Biotinylated recombinant human MUC-1 protein (Ser24-Ser380) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Form
Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 62 kDa
SDS-PAGE: 168-195 kDa, under reducing conditions
Protein length
Ser24-Ser380
Bio-activity
Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human MUC-1 Fc Chimera Avi-tag is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Siglec-9 Fc Chimera binds with an ED50 of 0.25-2 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human MUC-1 Fc Chimera Avi-tag is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Siglec-9 Fc Chimera binds with an ED50 of 0.25-2 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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