Recombinant Human MYDGF Protein (33-173aa), N-His-Tagged
Product Description
Cat
IMP-9546
Official Symbol
MYDGF
Product Overview
Recombinant human SF20/IL25 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.
Description
SF20/25, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown.
Expression System
E. coli
Species
Human
Tag
N-His
Form
Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Molecular Mass
18 kDa (162 aa) confirmed by MALDI-TOF
Protein length
33-173
AA Sequence
<MGSSHHHHHHSSGLVPRGSHM>SEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAVAHRPGAFKAELSKLVIVAKASRTEL
Purity
> 95% by SDS-PAGE
Applications
SDS-PAGE
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by Bradford assay)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info