Recombinant Human NECTIN1 Protein (Gln31-Gly346), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10049
Official Symbol
NECTIN1
Product Overview
Biotinylated recombinant human Nectin-1 protein (Gln31-Gly346) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Gln31 inferred from deblocking revealing Val32
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 64 kDa
SDS-PAGE: 85-96 kDa, under reducing conditions
Protein length
Gln31-Gly346
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 Protein is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human Nectin-1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 6.0-40 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 Protein is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human Nectin-1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 6.0-40 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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