Recombinant Human NECTIN1 Protein (Gln31-Gly346), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10049
Official Symbol
NECTIN1
Product Overview
Biotinylated recombinant human Nectin-1 protein (Gln31-Gly346) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Gln31 inferred from deblocking revealing Val32
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 64 kDa
SDS-PAGE: 85-96 kDa, under reducing conditions
Protein length
Gln31-Gly346
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 Protein is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human Nectin-1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 6.0-40 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 Protein is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human Nectin-1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 6.0-40 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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