Recombinant Human NECTIN2 Protein, C-hFc& AVI-tagged, Biotinylated

Product Description

Cat

IMP-1395

Official Symbol

NECTIN2

Product Overview

Recombinant human NECTIN2 (NP_002847.1)(Met1-Leu360) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Description

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell-and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Expression System

HEK293

Species

Human

Tag

C-hFc& AVI

Predicted N Terminal

Gln 32

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human NECTIN2 consists of 582 amino acids and predicts a molecular mass of 64.08 kDa. It migrates as an approximately 75.99 kDa band in SDS-PAGE under reducing conditions.

Protein length

Met1-Leu360

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized Human PVRIG hFc at 2 μg/mL (100 μL/well) can bind Human Nectin-2 (ECD, hFc&AVI Tag), Biotinylated), the EC50 of Human Nectin-2 (ECD, hFc&AVI Tag), Biotinylated is 10-60 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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