Recombinant Human NECTIN2 protein, C-His Tag
Product Description
Cat
IMP-245
Official Symbol
NECTIN2
Product Overview
Recombinant Human NECTIN2 protein(AA Gln 32-Leu 360)(Accession # Q92692-2) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-His Tag
Form
Lyophilized from sterile PBS, pH7.4, 10% trehalose.
Molecular Mass
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.4 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Gln 32-Leu 360
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human Nectin-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human Nectin-2, His Tag with a linear range of 0.078-0.625 ug/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Human TIGIT, Fc Tag at 10 ug/mL (100 uL/well) can bind Human Nectin-2, His Tag with a linear range of 0.625-5 ug/mL.

Bioactivity-ELISA 3
Bioactivity-ELISA

Immobilized Human PVRIG, Fc Tag at 5 ug/mL (100 uL/well) can bind Human Nectin-2, His Tag with a linear range of 0.039-0.625 ug/mL.

Bioactivity-BLI 1

Loaded Human PVRIG, Fc Tag on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.61 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2
Bioactivity-BLI

Loaded Rat TIGIT, Fc Tag on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.59 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3
Bioactivity-BLI

Loaded Human DNAM-1, Fc Tag on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.66 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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