Recombinant Human NECTIN2 protein, C-mFc Tag

Product Description

Cat

IMP-118

Official Symbol

NECTIN2

Product Overview

Recombinant Human NECTIN2 protein(AA Gln 32-Leu 360)(Accession # Q92692-2) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 62.4 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 32-Leu 360

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human Nectin-2, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human DNAM-1, Fc Tag at 5 ug/mL (100 uL/well) can bind Human Nectin-2, Mouse IgG2a Fc Tag with a linear range of 2-20 ng/mL.

Bioactivity-ELISA 2

Immobilized Biotinylated Human PVRIG, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, bind Human Nectin-2, Mouse IgG2a Fc Tag with a linear range of 0.1-5 ng/mL.

Bioactivity-BLI 1

Loaded Human Nectin-2, Mouse IgG2a Fc Tag on Protein A Biosensor, can bind Human PVRIG, His Tag with an affinity constant of 1.33 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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