Recombinant Human NECTIN3 protein, C-Fc Tag

Product Description

Cat

IMP-242

Official Symbol

NECTIN3

Product Overview

Recombinant Human NECTIN3 protein(AA Gly 58-Asp 400)(Accession # Q9NQS3) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gly 58-Asp 400

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human Nectin-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human Nectin-1, His Tag at 2 ug/mL (100 uL/well) can bind Human Nectin-3, Fc Tag with a linear range of 5-78 ng/mL.

Bioactivity-ELISA 2

Immobilized Human TIGIT, His Tag at 1 ug/mL (100 uL/well) can bind Human Nectin-3, Fc Tag with a linear range of 0.156-2.5 ug/mL.

Bioactivity-BLI 1

Loaded Human Nectin-3, Fc Tag on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.732 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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