Recombinant Human NECTIN3 Protein (Leu56-Asp400 & Gly58-Asp400), C-6×His-tagged
Product Description
Cat
IMP-9750
Official Symbol
NECTIN3
Product Overview
Recombinant human Nectin-3 protein (Leu56-Asp400 & Gly58-Asp400) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Leu56&Gly58
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 38.7 kDa
SDS-PAGE: 66-85 kDa, reducing conditions
Protein length
Leu56-Asp400 & Gly58-Asp400
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized rhNectin-3/his at 1 μg/mL can bind rhNectin-1/his with an apparent KD <120 nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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