Recombinant Human NECTIN4 Protein, C-His-Avi tag, Biotinylated

Product Description

Cat

IMP-974

Official Symbol

NECTIN4

Product Overview

Biotinylated Recombinant Human NECTIN4(Q96NY8)(Gly32-Val351) Protein, fused with a 6*His, Avi tag at the C-terminus, is expressed from HEK293 cells.

Expression System

HEK293

Species

Human

Tag

C-His-Avi tag

Form

Lyophilized from sterile 20mM PB, 150mM NaCl, pH 7.4.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 37.2kDa, and migrates as 40-50kDa under reducing conditions.

Protein length

Gly32-Val351

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Notes

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human Nectin-4-His-Avi at 2μg/ml (100 μl/well) can bind Anti-Human Nectin-4 mAb. The ED50 of Anti-Human Nectin-4 mAb is 12.28 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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