Recombinant Human NECTIN4 protein, C-His Tag

Product Description

Cat

IMP-5990

Official Symbol

NECTIN4

Product Overview

Recombinant Human NECTIN4 protein(Q96NY8)(32-351aa), fused with C-terminal His tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4

Molecular Mass

35.3 kDa

Protein length

32-351aa

AA Sequence

GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVV

Endotoxin

Less than 1.0 EU/µg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Biological Activity

Specific activity as determined by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells is greater than 40%. When 5 x 10^4 cells/well are added to plates coated with recombinant human Nectin-4 (1 µg/mL with 100 µL/well), approximately >50% will adhere after 40 min incubation at 37 °C.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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