Recombinant Human NEDD8 Protein (1-76), His-tagged
Product Description
Cat
IMP-6917
Official Symbol
NEDD8
Product Overview
Recombinant human NEDD8 (1-76) protein with His tag was expressed in E. coli.
Description
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.[UniProtKB/Swiss-Prot Function]
Expression System
E. coli
Species
Human
Tag
His
Form
Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.5 mM DTT, 50 mM NaCl, 10% glycerol
Molecular Mass
12.8 kDa
Protein length
1-76
AA Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHMLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method
Purity
> 95% by SDS-PAGE.
Storage
Store undiluted at 2-8 centigrade for up to two weeks or (in aliquots) at -20 or -70 centigrade for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Concentration
lot specific
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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