Recombinant Human NEFL protein, C-hFc Tag

Product Description

Cat

IMP-3210

Official Symbol

NEFL

Product Overview

Recombinant Human NEFL protein(9-88aa), fused with C-terminal hFc Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-hFc Tag

Form

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Molecular Mass

The protein has a predicted molecular mass of 34.9 kDa after removal of the signal peptide. The apparent molecular mass of NEFL(9-88)-hFc is approximately 35-55 kDa due to glycosylation.

Protein length

9-88aa

Purity

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Biological Activity

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human NEFL (9-88) Protein, hFc Tag can bind Anti-NEFL(9-88) antibody, Rabbit mAb in a linear range of 0.128-80 ng/mL.

Storage

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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