Recombinant Human NFKBIA Protein (1-317), N-GST-tagged

Product Description

Cat

IMP-8673

Official Symbol

NFKBIA

Product Overview

Human NFKBIA full-length ORF ( AAH04983, 1 a.a.-317 a.a.) recombinant protein with GST-tag at N-terminal was expressed in Wheat Germ (in vitro).

Description

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Expression System

Wheat Germ (in vitro)

Species

Human

Tag

N-GST

Form

50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Molecular Mass

60.61 kDa

Protein length

1-317

AA Sequence

MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL

Applications

ELISA; WB (Recombinant protein); Antibody Production; Protein Array

Notes

Best use within three months from the date of receipt of this protein.

Storage

Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

SDS-PAGE

12.5% SDS-PAGE Stained with Coomassie Blue.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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