IMP-9782

NOTCH2

Recombinant human Notch-2 protein (Leu26-Gln530) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

CHO

Human

C-hFc

Leu26

Lyophilized from a 0.2 μm filtered solution in PBS.

Predicted Molecular Mass: 80.6 kDa (monomer)
SDS-PAGE: 115 kDa, reducing conditions

Leu26-Gln530

Measured by its binding ability in a functional ELISA. Immobilized rhNotch-2/Fc Chimera can bind rrJagged-1/Fc Chimera with an apparent KD <10 nM.

<0.01 EU/μg of the protein by the LAL method.

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Disulfide-linked homodimer

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitute at 500 μg/mL in sterile PBS.